I would think once it loads it's in the cache. So, when the second location is activated there would be no need to "tell" tourweaver to "reload".
Your .swf or .flv will load in the background but depending on file size may not fully be loaded when called upon. Works a lot like Youtube.
I'm working on updating a project that may help with these questions.
Link To Tour
Let the tour play through the first scene (porch). It then goes into the second scene (living). Here you will see that the .flv loads but is still not fully loaded. Again a lot like a video on Youtube. It opens the second scene but still needs to buffer a little for the .flv on the tele.
Same .flv is pulled through the "Welcome" button at the top of the screen. Same as what your referring to with one file being used in two locations.
Hope that helps. I know there are others that can better answer but thought I would at least try.